lipofectamine 3000 transfection ar ti cl e (ATCC)
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Lipofectamine 3000 Transfection Ar Ti Cl E, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 12140 article reviews
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Control:Article Title: miR-671-5p as a diagnostic biomarker and therapeutic target in periodontitis via THBS1 regulation. Article Snippet: Amplified sequences were cloned into the pmirGLO dual-luciferase vector (Promega, USA) to generate wildtype (WT-THBS1, containing the miR-671-5p binding site) and mutant (MT-THBS1, with a mutated binding site) reporter constructs for the THBS1 3’UTR. .. These WT or MT vectors were co-transfected with miR-671-5p mimic or control into hPDLFs (ATCC, USA) using Article Title: miR-671-5p as a diagnostic biomarker and therapeutic target in periodontitis via THBS1 regulation Article Snippet: Amplified sequences were cloned into the pmirGLO dual-luciferase vector (Promega, USA) to generate wild-type (WT-THBS1, containing the miR-671-5p binding site) and mutant (MT-THBS1, with a mutated binding site) reporter constructs for the THBS1 3’UTR. .. These WT or MT vectors were co-transfected with miR-671-5p mimic or control into hPDLFs (ATCC, USA) using Transfection:Article Title: miR-671-5p as a diagnostic biomarker and therapeutic target in periodontitis via THBS1 regulation. Article Snippet: Amplified sequences were cloned into the pmirGLO dual-luciferase vector (Promega, USA) to generate wildtype (WT-THBS1, containing the miR-671-5p binding site) and mutant (MT-THBS1, with a mutated binding site) reporter constructs for the THBS1 3’UTR. .. These WT or MT vectors were co-transfected with miR-671-5p mimic or control into hPDLFs (ATCC, USA) using Article Title: Neural synaptic vesicle autoimmunity following aerosolized porcine neural tissue exposure: insights into autoimmune inflammatory polyradiculoneuropathy. Article Snippet: .. COS7 cells (ATCC, #CRL-1651) were transiently transfected ( Article Title: RNA-editing oligonucleotides and uses thereof Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Article Title: A combination study of benzyloxyphenyl NCX inhibitor and its insensitive Slc8a1 mutation knock-in mouse reveals NCX1-specific physiological functions. Article Snippet: The Na+/Ca2+ exchanger (NCX) is a bidirectional transporter that is controlled by membrane potential and transmembrane gradients of Na and Ca2+.. Benzyloxyphenyl NCX inhibitors such as SEA0400 and KB-R7943 are widely used for NCX research.. However, since these inhibitors have shown to possess somewhat nonspecific actions, it is necessary to discriminate between the specific and nonspecific actions in their pharmacological experiments. Article Title: Neural synaptic vesicle autoimmunity following aerosolized porcine neural tissue exposure: insights into autoimmune inflammatory polyradiculoneuropathy Article Snippet: .. COS7 cells (ATCC, #CRL-1651) were transiently transfected ( Article Title: miR-671-5p as a diagnostic biomarker and therapeutic target in periodontitis via THBS1 regulation Article Snippet: Amplified sequences were cloned into the pmirGLO dual-luciferase vector (Promega, USA) to generate wild-type (WT-THBS1, containing the miR-671-5p binding site) and mutant (MT-THBS1, with a mutated binding site) reporter constructs for the THBS1 3’UTR. .. These WT or MT vectors were co-transfected with miR-671-5p mimic or control into hPDLFs (ATCC, USA) using Plasmid Preparation:Article Title: Neural synaptic vesicle autoimmunity following aerosolized porcine neural tissue exposure: insights into autoimmune inflammatory polyradiculoneuropathy. Article Snippet: .. COS7 cells (ATCC, #CRL-1651) were transiently transfected ( Article Title: RNA-editing oligonucleotides and uses thereof Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Article Title: Bat organoids reveal antiviral responses at epithelial surfaces Article Snippet: SINV encoding eGFP and harboring a mutation in the nsP2 gene (P726G) was constructed using PCR site-directed mutagenesis of the pBG218 rescue plasmid (Kerafast) encoding a replication-competent SINV genome. .. Virus rescue was carried out by transfecting 70% confluent BHK-21 cells (ATCC) with the pBG218-nsP2-P726G plasmid using Article Title: Neural synaptic vesicle autoimmunity following aerosolized porcine neural tissue exposure: insights into autoimmune inflammatory polyradiculoneuropathy Article Snippet: .. COS7 cells (ATCC, #CRL-1651) were transiently transfected ( Clone Assay:Article Title: A combination study of benzyloxyphenyl NCX inhibitor and its insensitive Slc8a1 mutation knock-in mouse reveals NCX1-specific physiological functions. Article Snippet: The Na+/Ca2+ exchanger (NCX) is a bidirectional transporter that is controlled by membrane potential and transmembrane gradients of Na and Ca2+.. Benzyloxyphenyl NCX inhibitors such as SEA0400 and KB-R7943 are widely used for NCX research.. However, since these inhibitors have shown to possess somewhat nonspecific actions, it is necessary to discriminate between the specific and nonspecific actions in their pharmacological experiments. Construct:Article Title: A combination study of benzyloxyphenyl NCX inhibitor and its insensitive Slc8a1 mutation knock-in mouse reveals NCX1-specific physiological functions. Article Snippet: The Na+/Ca2+ exchanger (NCX) is a bidirectional transporter that is controlled by membrane potential and transmembrane gradients of Na and Ca2+.. Benzyloxyphenyl NCX inhibitors such as SEA0400 and KB-R7943 are widely used for NCX research.. However, since these inhibitors have shown to possess somewhat nonspecific actions, it is necessary to discriminate between the specific and nonspecific actions in their pharmacological experiments. Polymerase Chain Reaction:Article Title: A combination study of benzyloxyphenyl NCX inhibitor and its insensitive Slc8a1 mutation knock-in mouse reveals NCX1-specific physiological functions. Article Snippet: The Na+/Ca2+ exchanger (NCX) is a bidirectional transporter that is controlled by membrane potential and transmembrane gradients of Na and Ca2+.. Benzyloxyphenyl NCX inhibitors such as SEA0400 and KB-R7943 are widely used for NCX research.. However, since these inhibitors have shown to possess somewhat nonspecific actions, it is necessary to discriminate between the specific and nonspecific actions in their pharmacological experiments. Virus:Article Title: Bat organoids reveal antiviral responses at epithelial surfaces Article Snippet: SINV encoding eGFP and harboring a mutation in the nsP2 gene (P726G) was constructed using PCR site-directed mutagenesis of the pBG218 rescue plasmid (Kerafast) encoding a replication-competent SINV genome. .. Virus rescue was carried out by transfecting 70% confluent BHK-21 cells (ATCC) with the pBG218-nsP2-P726G plasmid using Cell Culture:Article Title: Bat organoids reveal antiviral responses at epithelial surfaces Article Snippet: SINV encoding eGFP and harboring a mutation in the nsP2 gene (P726G) was constructed using PCR site-directed mutagenesis of the pBG218 rescue plasmid (Kerafast) encoding a replication-competent SINV genome. .. Virus rescue was carried out by transfecting 70% confluent BHK-21 cells (ATCC) with the pBG218-nsP2-P726G plasmid using Article Title: Ubiquitination Regulates Reorganization of the Membrane System During Cytomegalovirus Infection Article Snippet: Briefly, 5 μg of the pLIX HA-Ub lentiviral plasmid was mixed with 10 μg of p8.91 (a gift from Simon Davis; Addgene plasmid # 187441; http://n2t.net/addgene:187441 , accessed on 27 July 2025; RRID:Addgene_187441) [ ] and 0.5 μg of plasmid p-CMV-VSV-G (a gift from Bob Weinberg; Addgene plasmid #8454; http://n2t.net/addgene:8454 , accessed on 27 July 2025; RRID:Addgene_8454) [ ], in 1.5 mL Optimem (Thermo Fisher Scientific, Waltham, MA, USA; (Cat. No. 31-985-070). .. In parallel, 41 μL |
